Data points shown Figure S6 to calculate linear fits for viscosity determination Data sets for 5-DSPC in methanol:glycerol mixtures Viscosity in mPas Rotational correlation time in ns Segmental order parameter mean SD mean SD 0.63 0.15 0.01 0.519 0.031 14.03 1.40 0.07 0.538 0.032 30.73 2.54 0.13 0.562 0.034 72.97 4.54 0.23 0.602 0.036 183.43 7.28 0.36 0.650 0.039 Data sets for 12-DSPC in methanol:glycerol mixtures Viscosity in mPas Rotational correlation time in ns Segmental order parameter mean SD mean SD 0.63 0.14 0.01 0.263 0.021 14.03 0.94 0.05 0.271 0.022 30.73 1.90 0.10 0.289 0.023 72.97 3.50 0.18 0.313 0.025 183.43 5.50 0.28 0.345 0.028 Data sets for 16-DSPC in methanol:glycerol mixtures Viscosity in mPas Rotational correlation time in ns Segmental order parameter mean SD mean SD 0.63 0.10 0.01 0.107 0.009 14.03 0.40 0.02 0.120 0.010 30.73 0.80 0.04 0.145 0.012 72.97 1.58 0.08 0.184 0.015 183.43 2.75 0.14 0.236 0.019 Data sets for C10-BODIPY in methanol:glycerol mixtures Viscosity in mPas Fluorescence lifetime in ns mean SD 0.63 0.36 0.05 14.03 0.59 0.07 30.73 0.95 0.06 72.97 1.34 0.07 183.43 2.05 0.10 513.37 3.30 0.10